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KMID : 0380220070400020270
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Journal of Biochemistry and Molecular Biology
2007 Volume.40 No. 2 p.270 ~ p.276
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Cloning and Expression Analysis of a Novel Mouse Zinc Finger Protein Gene Znf313 Abundantly Expressed in Testis
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Li Na
Sun Huaqin
Wu Qiaqing
Tao Dachang
Zhang Sizhong
Ma Yongxin
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Abstract
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We have cloned a novel mouse zinc finger protein gene Znf313 by rapid amplification of cDNA ends (RACE) according to the homologue of human ZNF313 gene. The cDNA is 2,163 base pairs (bp) in length and encodes a 229 amino acids (aa) protein with a C3HC4 ring finger domain and three C2H2 domains. 89% and 93% nucleotide (nt) and aa sequence identity is observed with its human homologue. Revealed by Northern blot and RT-PCR, full mRNA consists of 2.16 kb and widely expresses in tissues as a single transcript, most abundantly in heart, liver, kidney and testis. The expression of Znf313 in testis is detected in all development stages. Western blot analysis also reveals that Znf313 is expressed in the tissues. Immunohistochemical staining and subcellular localization demonstrate that Znf313 is expressed both in the cytoplasm and nucleus whereas predominantly localized in the nucleus. Present data suggests that Znf313 gene might play a fundamental role in gene transcription and regulation in organism and relates to spermatogenesis.
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KEYWORD
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Mouse Znf313 gene, Northern blot, RACE, RTPCR, Subcellular localization
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